Circular Dichroism Spectroscopy Lab
In this lab we will first carry out a few simple experiments to understand the principle working behind CD spectroscopy. We will then proceed with experiments involving the CD spectrophotometer, which is by far the most effective of the three methods.
- To determine the specific rotation of a sugar using a polarimeter.
- To understand the effect of chiral substances on plane polarized light as a function of wavelength.
- To study the Optical Rotatory Dispersion (ORD) of some chiral substances.
- To study the Circular Dichroism of some substances with chiral chromophores.
- To Study the operational details of a CD spectrometer.
- To Study the effect of Secondary Structure elements on CD spectrum of proteins.
- To deconvolute the CD spectrum of a given protein solution and to classify it in terms of its secondary structure elements.
- To study the thermal stability of proteins.
- To study the effect of chemical denaturants on protein stability.
- To study the comparison between protein unfolding and protein aggregation in the presence of chemical denaturing agents.