To study the comparison between protein unfolding and protein aggregation in the presence of chemical denaturing agents
1. Video Tutorial
click [here](video/manual_exp10.swf) to ENLARGE.
2. Step-by-step Procedure
Step1 : Select any one of the two samples.
Step2 : Follow the instructions given in the Experiemntal-setup page.
Step3 : Select the temperature range and step size of your interest.
Step4 : Go through the instructions and fill up the table by clicking at the 222nm.
Step5 : Observe the plot and find out the transition temperature.
Step6 : Click on the "Measure Dynode Voltage".
Step7 : Follow the instruction and fill up the table by clicking at the 222nm.
Step8 : Observe the change in Dynode voltage.
Step9 : Click on "Go to Analysis"
Step 10 : Submit your answers. You can verify them from redoing the experiment or going through the theory.
Step 11 : Once you have studied both the samples you may compare them by clicking "COMPARE".